Physiological Studies on the Germination of Bacillus subtilis Spores
نویسندگان
چکیده
منابع مشابه
Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.
Bacillus subtilis spores prepared in rich medium germinated faster with nutrient germinants than poor-medium spores as populations in liquid and multiple individual spores on a microscope slide. Poor-medium spores had longer average lag times between mixing of spores with nutrient germinants and initiation of Ca-dipicolinic acid (CaDPA) release. Rich-medium spores made at 37°C germinated slight...
متن کاملRole of GerD in germination of Bacillus subtilis spores.
Spores of a Bacillus subtilis strain with a gerD deletion mutation (Delta gerD) responded much slower than wild-type spores to nutrient germinants, although they did ultimately germinate, outgrow, and form colonies. Spores lacking GerD and nutrient germinant receptors also germinated slowly with nutrients, as did Delta gerD spores in which nutrient receptors were overexpressed. The germination ...
متن کاملUltrastructural analysis during germination and outgrowth of Bacillus subtilis spores.
Electron microscopy of thin sections of dormant and germinating spores of Bacillus subtilis 168 revealed a progressive change in the structure of the cortex, outer spore coat, and inner spore coat. The initial changes were observed in the cortex region, which showed a loose fibrous network within 10 min of germination, and in the outer spore coat, which began to be sloughed off. The permeabilit...
متن کاملChromosomes in Bacillus subtilis spores and their segregation during germination.
Spores of a thymine-requiring mutant of Bacillus subtilis 168 leucine(-), indole(-), thymine(-)) were uniformly labeled with (3)H-thymidine. These were seeded on thinlayer agar plates where they germinated into long-chained microcolonies. Autoradiograms were used to measure the distribution of labeled deoxyribonucleic acid in the chains of cells, which ranged in length from 2 to 32 cells. Four ...
متن کاملAnalysis of the effects of a gerP mutation on the germination of spores of Bacillus subtilis.
As previously reported, gerP Bacillus subtilis spores were defective in nutrient germination triggered via various germinant receptors (GRs), and the defect was eliminated by severe spore coat defects. The gerP spores' GR-dependent germination had a longer lag time between addition of germinants and initiation of rapid release of spores' dipicolinic acid (DPA), but times for release of >90% of ...
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ژورنال
عنوان ژورنال: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
سال: 1963
ISSN: 0015-6426,1882-1006
DOI: 10.3358/shokueishi.4.32